TC Acclimating

Tissue Culture Acclimation Schedule

 (Fresh Out of Flask)

• Rinse: Carefully wash off agar with lukewarm distilled or clean water.
  
  
• Medium: Plant in a fine, airy, sterile mix (sphagnum moss, perlite, or aroid mix depending on species).
  
  
• Humidity: 95–100% (use a sealed clear container, plastic dome, or zip-lock bag).
  
  
• Light: Bright indirect light, 10–12 hours (avoid direct sun).
  
  
• Temperature: 75–80°F (24–27°C).
  
  
• Water: Keep medium moist, not soggy.
  
  

Week 1

• Start venting the container: open lid/zipper for 10–15 minutes once or twice a day.
  
  
• Humidity stays high (90–95%).
  
  
• Watch for leaf wilt — if severe, reduce venting.
  
  

Week 2

• Increase venting gradually: 30–60 minutes twice daily.
  
  
• Humidity ~85–90%.
  
  
• Plants start thickening leaves, roots begin adapting.
  
  
• Maintain bright indirect light.
  
  

Week 3

• Leave lids cracked or partially open most of the day.
  
  
• Humidity ~75–85%.
  
  
• By now plants should show some growth (new leaves, root tips extending).
  
  

Week 4

• Move to open air in grow tent or greenhouse conditions.
  
  
• Humidity ~70–75% (good for most tropicals).
  
  
• Water normally, allow medium surface to dry slightly between waterings.
  
  
• Begin light fertilization (¼ strength).
  
  

After Week 4

• Plants are usually hardened off enough to handle “normal” aroid or houseplant care.
  
  
• Gradually adapt them to your final environment (room humidity or greenhouse).
  
  
• Avoid direct sun until they are stronger.
  
  

⚠️ Tips & Warnings:

• Some species (like anthuriums, alocasias, philodendrons) may need longer hardening.
  
  
• If you see collapse/wilting, increase humidity again and slow down.
  
  
• Don’t fertilize too early — roots are sensitive at first.